Fgf21 Conditional Knockout mouse models
Fgf21, the mouse ortholog of human FGF21, encodes an endocrine FGF whose weak heparan sulfate affinity permits circulation and whose activity requires the coreceptor beta-Klotho paired with FGFR1c on adipocytes, or FGFR1c in hypothalamus and hindbrain. Hepatic transcription is induced by PPARalpha during fasting and ketogenesis and by ATF4 during amino acid restriction and integrated stress response, and the protein raises adipose glucose uptake and adiponectin, promotes energy expenditure, and centrally suppresses sweet and alcohol preference while stimulating water intake. Circulating levels are limited by fibroblast activation protein cleavage, a fact exploited by protease-resistant analogs including efruxifermin and pegozafermin. Nulls are viable, so constitutive deletion suits metabolic challenge protocols, while conditional floxed alleles distinguish hepatocyte-derived from adipose or muscle sources and Klb conditionals localize the responsive tissue. No paralog substitutes because beta-Klotho dependence is specific, though FGF15 and FGF19 share the coreceptor with FGFR4. Humanized knockins are the appropriate platform for analog pharmacokinetics and receptor-selectivity endpoints.
Fgf21 conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype.
What Fgf21 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Fgf21 conditional knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Fgf21 Conditional Knockout allele
Since nulls survive, the conditional allele exists to answer a source question, not a viability question. Flanking the exons that encode the mature secreted peptide, including the carboxy-terminal segment that docks beta-Klotho, removes bioactivity without leaving a signal peptide fragment that could still be secreted. Alb-Cre removes the hepatocyte pool that dominates fasting and ketogenic plasma levels, but recombination completes only after birth, so early developmental contributions escape. Adipoq-Cre addresses the autocrine adipose pool, Ckmm-Cre the exercise and mitochondrial stress pool, and Ptf1a-Cre the acinar pool that confuses whole-body interpretation. Pair each with a Klb conditional on the receiving side, because deleting the ligand cannot distinguish adipose from hindbrain responsiveness. Measure plasma protein to confirm compartment loss, then read fasting ketogenesis, adiponectin, energy expenditure, and sucrose preference.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Fgf21 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgf21 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgf21 Conditional Knockout mouse models are available?
When Fgf21 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgf21 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgf21 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgf21 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgf21?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.