Flt3 Conditional Knockout mouse — endothelial specific context
FMS-like tyrosine kinase 3, encoded by Flt3 and orthologous to human FLT3, is a class III receptor tyrosine kinase with five immunoglobulin-like extracellular domains, a juxtamembrane autoinhibitory segment, and a split kinase domain, driving STAT5, RAS/MAPK, and PI3K signaling upon FLT3 ligand binding on multipotent and lymphoid progenitors. Internal tandem duplications within the juxtamembrane region relieve autoinhibition and impose constitutive, mislocalized STAT5 activation, while tyrosine kinase domain substitutions at D835 confer a distinct active conformation and differing sensitivity to type I versus type II inhibitors. Because a straightforward null is viable with only modest progenitor deficits, informative cancer models use knockin ITD alleles, which produce myeloproliferative disease and cooperate with Npm1 or Dnmt3a mutations to yield frank leukemia. Ectodomain humanization supports antibody, bispecific, and CAR-T evaluation. Select allele class according to whether the endpoint is inhibitor resistance, cooperating mutation genetics, or immunotherapy targeting.
Flt3 conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype. For endothelial work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on endothelial while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Flt3-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 231301 | Inquire |
Pricing and quotes
The Flt3 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Flt3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Flt3 Conditional Knockout mouse models are available?
When Flt3 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Flt3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Flt3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for endothelial specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to endothelial specific, so we start driver selection there.
Do you ship live Flt3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Flt3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.