Foxo1 Conditional Knockout mouse models
Foxo1, the mouse ortholog of human FOXO1, encodes a forkhead box O transcription factor that binds the DAF-16 family consensus element through a winged helix domain and activates gluconeogenic, autophagy, cell cycle arrest and stress resistance programs including G6pc, Pck1, Cdkn1b, Gadd45a and Sod2. Insulin and growth factor signaling suppress it directly, since AKT phosphorylates threonine 24, serine 253 and serine 316 to create 14-3-3 binding sites that drive nuclear exclusion and SKP2 dependent degradation, while SIRT1 deacetylation and JNK phosphorylation reverse that control under stress. PAX3-FOXO1 and PAX7-FOXO1 fusions from the t(2;13) and t(1;13) translocations define alveolar rhabdomyosarcoma, a distinct oncogenic use of this locus. Null embryos die at midgestation from vascular remodeling failure, so conditional alleles are required, and a triple alanine knockin that resists AKT phosphorylation gives constitutive nuclear activity for insulin resistance studies. Foxo3 and Foxo4 compensate substantially, so compound conditional alleles are needed for hematopoietic and metabolic endpoints.
Tissue restricted knockout of Foxo1 keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Foxo1 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Foxo1 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Foxo1-Flox | Conditional Knockout | KO/CKO mice, disease model mice | live | CKO 200177 | Inquire |
Designing a Foxo1 Conditional Knockout allele
Because germline nulls die near midgestation from failed vascular remodeling, tissue-restricted deletion is the only route to adult biology at this locus. Flank the exons encoding the forkhead DNA-binding domain rather than the transactivation tail, so any residual product cannot occupy DAF-16 family elements and cannot act as a dominant negative on Foxo3. Match driver to compartment: Alb-Cre or Alb-CreERT2 for hepatic gluconeogenesis, Ins1-Cre rather than older RIP-Cre lines that carry human growth hormone sequence and distort islet mass, Cdh5-CreERT2 for endothelial questions that recapitulate the lethality in adults, Vav-Cre or Mx1-Cre for hematopoietic stem cells. Expect partial phenotypes wherever Foxo3 and Foxo4 are coexpressed, so plan compound floxed alleles from the start. Read pyruvate tolerance, hepatic G6pc and Pck1 transcripts, fasting insulin, and stem cell quiescence by label retention.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Foxo1 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Foxo1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Foxo1 Conditional Knockout mouse models are available?
When Foxo1 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Foxo1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Foxo1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Foxo1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Foxo1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.