Foxo1 Knockout mouse models
Foxo1, the mouse ortholog of human FOXO1, encodes a forkhead box O transcription factor that binds the DAF-16 family consensus element through a winged helix domain and activates gluconeogenic, autophagy, cell cycle arrest and stress resistance programs including G6pc, Pck1, Cdkn1b, Gadd45a and Sod2. Insulin and growth factor signaling suppress it directly, since AKT phosphorylates threonine 24, serine 253 and serine 316 to create 14-3-3 binding sites that drive nuclear exclusion and SKP2 dependent degradation, while SIRT1 deacetylation and JNK phosphorylation reverse that control under stress. PAX3-FOXO1 and PAX7-FOXO1 fusions from the t(2;13) and t(1;13) translocations define alveolar rhabdomyosarcoma, a distinct oncogenic use of this locus. Null embryos die at midgestation from vascular remodeling failure, so conditional alleles are required, and a triple alanine knockin that resists AKT phosphorylation gives constitutive nuclear activity for insulin resistance studies. Foxo3 and Foxo4 compensate substantially, so compound conditional alleles are needed for hematopoietic and metabolic endpoints.
Whole body loss of Foxo1 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Foxo1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Foxo1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Foxo1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 205036 | Inquire |
Designing a Foxo1 Knockout allele
A straight null is not a viable adult model, and the honest use of the allele is embryonic and cell-based. Homozygotes arrest with disorganized branchial arch and yolk sac vasculature, so the phenotype reports endothelial dosage rather than metabolism, and any metabolic question needs the floxed allele instead. The allele still earns its place. Heterozygotes are viable, and haploinsufficiency improves insulin sensitivity and attenuates diabetes in Irs2 deficient crosses, which makes the single-copy state a genetic modifier tool rather than a control. Null blastocysts and embryonic fibroblasts give clean systems for autophagy flux, Cdkn1b induction, and oxidative stress resistance without Cre variability. Delete across the forkhead exons and confirm loss by immunoblot rather than transcript, since truncated species accumulate. Score lethality timing, vessel morphology, and compensatory Foxo3 and Foxo4 expression.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Foxo1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Foxo1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Foxo1 Knockout mouse models are available?
When Foxo1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Foxo1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Foxo1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Foxo1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Foxo1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.