Foxo3 Knockout mouse models
Foxo3 is the mouse ortholog of human FOXO3 and encodes the forkhead factor most closely tied to oxidative stress resistance, quiescence and longevity, binding the same consensus element as FOXO1 and inducing Sod2, Cat, Gadd45a, Bcl2l11 encoding BIM, and Cdkn1b, thereby setting apoptotic threshold and stem cell reserve. Regulation mirrors the family, with AKT and SGK phosphorylation creating 14-3-3 docking sites for nuclear export, AMPK phosphorylation enhancing activity under energy stress, and IKK and ERK driving degradation. FOXO3 intronic haplotypes are among the most reproducible human longevity associations, and the gene is essential for primordial follicle quiescence, since null females exhaust the ovarian reserve and develop premature ovarian failure. Nulls are viable, so constitutive alleles support aging, hematopoietic stem cell and fertility studies directly. Because Foxo1 and Foxo4 occupy overlapping targets, single deletion underestimates requirement and compound conditional alleles are the standard for hematopoietic and neural stem cell work. Use phospho-resistant knockins to force nuclear retention.
Whole body loss of Foxo3 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Foxo3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Foxo3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Foxo3-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 190084 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Foxo3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Foxo3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Foxo3 Knockout mouse models are available?
When Foxo3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Foxo3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Foxo3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Foxo3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Foxo3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.