Gabra5 Knockout mouse models
Extrasynaptic inhibition in hippocampus depends heavily on the α5 subunit encoded by Gabra5, ortholog of human GABRA5, which assembles as α5βγ2 receptors anchored perisynaptically on CA1 pyramidal cell dendrites by radixin and generates a substantial component of tonic conductance that raises the threshold for dendritic integration and long-term potentiation. Because α5 receptors retain benzodiazepine site sensitivity, α5-selective inverse agonists enhance learning in rodents and have been pursued for cognitive deficits in Down syndrome, schizophrenia and post-stroke recovery, while α5 mediates part of the persistent memory impairment following etomidate and other general anesthetics. The gene lies in the imprinted 15q11 to q13 cluster with GABRB3 and GABRG3, adjacent to the Angelman and Prader-Willi interval. Constitutive nulls are viable with improved spatial learning, so a conditional null with Camk2a-Cre or Grik4-Cre assigns regional contribution, and a benzodiazepine-insensitive knockin is the allele matched to inverse agonist pharmacology.
A conventional Gabra5 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Gabra5 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gabra5 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gabra5-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 190573 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gabra5 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gabra5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gabra5 Knockout mouse models are available?
When Gabra5 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gabra5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gabra5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gabra5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gabra5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.