Gabra1 Knockout mouse models
Gabra1 encodes the α1 subunit of the GABA-A receptor, ortholog of human GABRA1, and is the most abundant α isoform in brain, assembling predominantly as α1β2γ2 pentamers at synapses where the benzodiazepine site is formed at the α1 and γ2 interface and requires His101 for high-affinity diazepam binding. Dominant human variants including A322D and pore-lining substitutions cause juvenile myoclonic epilepsy and developmental and epileptic encephalopathy through dominant negative assembly failure and reduced surface receptor rather than simple haploinsufficiency. The classic knockin H101R, which renders α1 diazepam-insensitive, remains the definitive demonstration that this subunit mediates the sedative and amnestic components of benzodiazepine action, and it exemplifies the point-mutation strategy for dissecting pharmacology. Constitutive nulls are viable with tremor and near-total loss of brain benzodiazepine binding, so choose a conditional null for circuit assignment, a patient knockin for encephalopathy modeling, and pair either with EEG and interneuron-specific Cre drivers.
A conventional Gabra1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Gabra1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gabra1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gabra1-KO | Knockout | KO/CKO mice, disease model mice | embryo cryopreservation | KO 190570 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gabra1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gabra1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gabra1 Knockout mouse models are available?
When Gabra1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gabra1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gabra1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gabra1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gabra1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.