Gabrd Knockout mouse models
The δ subunit encoded by Gabrd, ortholog of human GABRD, never occupies synapses; it replaces γ2 in extrasynaptic α4βδ and cerebellar α6βδ pentamers that mediate tonic inhibitory conductance, showing high GABA affinity, minimal desensitization, insensitivity to benzodiazepines and exceptional sensitivity to neurosteroids including allopregnanolone and to the superagonist gaboxadol. Expression is dynamically regulated across the ovarian cycle, pregnancy and the postpartum period, providing the mechanistic basis for catamenial and postpartum seizure susceptibility and for the therapeutic action of brexanolone and ganaxolone. Human variants including E177A and R220H associate with generalized epilepsy with febrile seizures plus and juvenile myoclonic epilepsy through reduced surface expression and channel open time. Constitutive nulls are viable with attenuated neurosteroid responses and altered seizure thresholds, making them directly useful; add a conditional null with a dentate or thalamic driver for circuit resolution, and note that Gabra4 deletion produces overlapping loss of tonic current.
A conventional Gabrd knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Gabrd Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gabrd knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gabrd-KO | Knockout | KO/CKO mice | live | KO 200604 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gabrd Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gabrd allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gabrd Knockout mouse models are available?
When Gabrd Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gabrd knockout embryonic lethal in mice?
It depends on background and allele design. Some Gabrd germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gabrd animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gabrd?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.