Gba Knockin mouse models
Gba, the mouse ortholog of human GBA1, encodes lysosomal acid beta-glucocerebrosidase, a retaining glycosidase that cleaves glucosylceramide into ceramide and glucose, depends on saposin C for activity, and reaches the lysosome via LIMP2 (Scarb2) rather than the mannose-6-phosphate route. Biallelic loss causes Gaucher disease with glucosylsphingosine accumulation, whereas single variant alleles such as N370S and L444P are the strongest common genetic risk factors for Parkinson disease, acting through impaired lysosomal flux and a reciprocal loop between reduced enzyme activity and alpha-synuclein aggregation. Constitutive nulls die within a day of birth from a defective epidermal permeability barrier, so conditional deletion with nestin-Cre or microglial drivers is required for adult neuropathology, while point knockins preserve residual activity and model heterozygous risk. The non-lysosomal paralog Gba2 hydrolyzes the same substrate elsewhere, so interpret lipid panels accordingly and match allele class to storage, chaperone response, or synucleinopathy endpoints.
An engineered Gba allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Gba Knockin mouse models are available?
ingenious targeting laboratory offers 3 distinct Gba knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Gba Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gba allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gba Knockin mouse models are available?
When Gba Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gba knockout embryonic lethal in mice?
It depends on background and allele design. Some Gba germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gba animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gba?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.