Gck Knockout mouse models
Glucokinase, encoded by Gck as the mouse ortholog of human GCK, is hexokinase IV, a monomeric enzyme with low glucose affinity, sigmoidal kinetics, no product inhibition by glucose-6-phosphate, and an allosteric activator site distinct from the catalytic cleft, properties that let it act as the glucose sensor of pancreatic beta cells and hepatocytes, where Gckr-encoded regulatory protein sequesters it in the nucleus until fructose-1-phosphate releases it. Heterozygous inactivating mutations produce GCK-MODY with a raised glucose threshold for insulin release, while activating mutations cause congenital hyperinsulinism, and homozygous null mice die perinatally from severe diabetes, so heterozygous or tissue-restricted conditional alleles are the workable designs. Beta-cell deletion using Ins1-Cre interrogates secretion thresholds, hepatocyte deletion using Alb-Cre interrogates glucose disposal and glycogen synthesis, activating knockins model hyperinsulinism, and humanization enables glucokinase activator pharmacology. Hexokinases 1 through 3 have far lower Km and cannot reconstitute sensing, so compensation is not a confound.
Global Gck deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Gck Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gck knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gck-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 251579 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gck Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gck allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gck Knockout mouse models are available?
When Gck Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gck knockout embryonic lethal in mice?
It depends on background and allele design. Some Gck germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gck animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gck?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.