Gli1 Knockin mouse models
As the mouse ortholog of human GLI1, Gli1 encodes a five-finger C2H2 zinc finger transcription factor that binds the GACCACCCA consensus and functions almost purely as an activator, lacking the strong amino-terminal repressor module of Gli3; it is itself a direct Hedgehog target gene, so its expression amplifies rather than initiates the pathway downstream of Ptch1 relief of Smoothened, with Sufu restraining nuclear entry. Because homozygous Gli1 nulls are viable and largely normal, the locus is exceptionally useful as a readout and lineage tool, and Gli1-LacZ or Gli1-CreER knockins report pathway activity and mark Hedgehog-responsive stroma, perivascular, and progenitor populations. Conditional nulls test tissue-autonomous requirements, while Rosa26-based activator expression asks whether forced GLI output suffices for transformation. Gli2 supplies most activator function and readily compensates, so single-gene loss frequently looks silent; compound Gli1 and Gli2 genetics, paired with a tamoxifen-controlled driver, keeps allele class matched to the endpoint.
Targeting Gli1 preserves positional context compared with viral or BAC approaches. That helps when you quantify expression or map chromatin.
What Gli1 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Gli1 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gli1-CreERT2 | Knockin | Cre/Dre Toolbox of Mice | live | KI 200073 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Gli1 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gli1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gli1 Knockin mouse models are available?
When Gli1 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gli1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gli1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gli1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gli1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.