Gli2 Knockout mouse models
Gli2, the mouse ortholog of human GLI2, encodes the principal transcriptional activator of Hedgehog signaling, a zinc finger protein bearing an amino-terminal repressor domain, a Sufu-binding region, and carboxy-terminal transactivation sequences; unlike Gli3, it is only inefficiently processed into a repressor form, and its activity is set by PKA-primed GSK3 and CK1 phosphorylation, Spop-directed degradation, and Kif7-dependent trafficking within the primary cilium. Homozygous nulls die perinatally with cleft palate, skeletal and vertebral defects, and lung and foregut abnormalities, so tissue-restricted floxed alleles are the practical route for postnatal or organ-specific questions. A conditional null asks whether a lineage requires activator output, an amino-terminally truncated constitutively active knockin asks whether GLI2 output alone drives proliferation or tumor initiation, and degron-resistant variants isolate turnover from transcription. Gli1 and Gli3 partially buffer loss, arguing for compound alleles, and a well-characterized Cre with confirmed recombination efficiency matters as much as the flox itself.
A conventional Gli2 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Gli2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gli2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gli2-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 251169 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gli2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gli2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gli2 Knockout mouse models are available?
When Gli2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gli2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gli2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gli2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gli2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.