READY TO SHIP!
IN PRODUCTION
Grin2d Conditional Knockout mouse models
Encoding GluN2D, Grin2d is the mouse ortholog of human GRIN2D and produces the NMDA receptor subunit with the slowest deactivation kinetics of the family, generating currents that decay over seconds and remain relatively resistant to magnesium block, properties that suit its enrichment in GABAergic interneurons, subthalamic nucleus, substantia nigra, and midbrain circuits. Expression peaks embryonically and in early postnatal life, then persists in defined inhibitory populations, where GluN2D-containing triheteromers shape sustained depolarization rather than brief coincidence detection. De novo GRIN2D variants such as V667I cause severe developmental and epileptic encephalopathy through gain of function, prolonging current and increasing agonist potency, so knockin alleles model the disease far better than deletion, which yields viable mice with modest behavioral change. Grin2c overlaps functionally in some populations and GluN2A or GluN2B substitutes elsewhere, so combine subunit-resolved recordings with interneuron-restricted Cre drivers and reserve humanized alleles for GluN2D-preferring modulator testing.
Conditional deletion of Grin2d limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Grin2d floxed stock yields usable cohorts.
What Grin2d Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Grin2d conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Grin2d-Flox | CKO | CKO | IN PRODUCTION | CKO 260382 | Inquire |
| These mice carry loxP sites flanking exon 7-8 of Grin2d gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Grin2d gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Grin2d Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Grin2d allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Grin2d Conditional Knockout mouse models are available?
When Grin2d Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Grin2d knockout embryonic lethal in mice?
It depends on background and allele design. Some Grin2d germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Grin2d animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Grin2d?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Grin2d Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.