Gsdmd Knockin mouse models
Gsdmd is the mouse ortholog of human GSDMD and encodes gasdermin D, a two-domain protein in which an autoinhibitory C-terminal domain restrains an N-terminal pore-forming module. Inflammatory caspase-1 and caspase-11, and in some settings caspase-8 or neutrophil elastase, cleave the interdomain linker at Asp276 in mouse, liberating the p30 fragment that binds acidic membrane lipids, oligomerizes into large beta-barrel pores, and provides the conduit for mature interleukin 1 beta and interleukin 18 export before NINJ1 dependent membrane rupture completes pyroptosis. Allele choice maps onto mechanism directly: a conditional null removes both cytokine release and lytic death, a D276A noncleavable knockin isolates processing from expression, and pore-dead point mutants separate lysis from unconventional secretion. Gsdme and the Gsdma cluster execute overlapping lytic programs downstream of caspase-3 or bacterial proteases, so pyroptosis-resistant phenotypes may require compound alleles, and myeloid restricted Cre lines assign the death to the intended lineage.
Gsdmd knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Gsdmd Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Gsdmd knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gsdmd-EGFP | Knockin | fluorescent mouse | sperm cryopreservation | KI 232075 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Gsdmd Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gsdmd allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gsdmd Knockin mouse models are available?
When Gsdmd Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gsdmd knockout embryonic lethal in mice?
It depends on background and allele design. Some Gsdmd germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gsdmd animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gsdmd?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.