Gsk3b Knockout mouse models
Encoding a constitutively active serine and threonine kinase that prefers substrates pre-phosphorylated four residues carboxy-terminal to its site, Gsk3b is the mouse ortholog of human GSK3B and is inhibited by Akt phosphorylation of Ser9 as well as by Wnt-driven sequestration; within the Axin and Apc destruction complex it phosphorylates beta-catenin at Ser33, Ser37, and Thr41 to license betaTrCP-mediated degradation, and it also targets tau, Snai1, Mcl1, Nfatc, and glycogen synthase. Homozygous nulls die at mid-gestation with hepatocyte apoptosis reflecting defective NF-kB survival signaling, so conditional deletion is standard, while a Ser9-to-alanine knockin creates an Akt-resistant kinase that tests whether inhibitory phosphorylation is required in vivo and a kinase-dead allele distinguishes scaffolding from catalysis. Critically, Gsk3a is a genuine paralog with overlapping substrate specificity, and many phenotypes emerge only in double conditional animals, so plan the compound cross and driver together with the primary flox.
A conventional Gsk3b knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Gsk3b Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gsk3b knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gsk3b-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 205058 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gsk3b Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gsk3b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gsk3b Knockout mouse models are available?
When Gsk3b Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gsk3b knockout embryonic lethal in mice?
It depends on background and allele design. Some Gsk3b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gsk3b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gsk3b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.