Havcr2 Conditional Knockout mouse models
Havcr2 encodes TIM-3, the mouse ortholog of human HAVCR2, an immunoglobulin V-type domain plus mucin stalk receptor expressed on exhausted CD8 T cells, Th1 cells, NK cells, and myeloid populations, with reported ligands including galectin 9, phosphatidylserine, CEACAM1, and HMGB1. Unlike PD-1 it carries no ITIM or ITSM, and instead its cytoplasmic tyrosines Y256 and Y263 are phosphorylated to displace HLA-B associated transcript 3 and permit Fyn dependent inhibitory signaling, which is why TIM-3 behaves as a context dependent regulator rather than a simple brake. Germline loss-of-function HAVCR2 variants cause subcutaneous panniculitis-like T cell lymphoma with hemophagocytic syndrome, giving a genuine disease knockin rationale distinct from a null. Because clinical antibodies do not cross-react, humanization is the allele for pharmacology, while conditional nulls with Cd4-Cre or Lyz2-Cre separate lymphoid from myeloid contributions, and combining with Pdcd1 loss is usually required before checkpoint phenotypes become measurable.
Conditional deletion of Havcr2 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Havcr2 floxed stock yields usable cohorts.
What Havcr2 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Havcr2 (TIM-3) conditional knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Havcr2 Conditional Knockout allele
TIM-3 does different jobs in different cells, so a floxed allele is the only way to assign a phenotype to a source. Flank the exon encoding the IgV domain, which removes phosphatidylserine, galectin 9 and CEACAM1 binding together, and leave the mucin stalk intact only if you confirm no membrane-anchored remnant still recruits Fyn. For the myeloid arm, Itgax-Cre addresses conventional dendritic cells and Lyz2-Cre the macrophage compartment, but neither is clean: Lyz2-Cre deletes poorly in dendritic cells while Itgax-Cre marks some macrophages and activated T cells, so pair them and confirm by sorting. Cx3cr1-CreER with tamoxifen suits microglia when neuroinflammation is the question, and E8I-Cre suits CD8-intrinsic exhaustion. Report deletion efficiency per sorted population, then read out cross-presentation and type I interferon for myeloid deletion, or tetramer-positive CD8 cytokine capacity and tumor burden for lymphoid deletion.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Havcr2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Havcr2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Havcr2 Conditional Knockout mouse models are available?
When Havcr2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Havcr2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Havcr2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Havcr2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Havcr2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.