Havcr2 Knockout mouse models
Havcr2 encodes TIM-3, the mouse ortholog of human HAVCR2, an immunoglobulin V-type domain plus mucin stalk receptor expressed on exhausted CD8 T cells, Th1 cells, NK cells, and myeloid populations, with reported ligands including galectin 9, phosphatidylserine, CEACAM1, and HMGB1. Unlike PD-1 it carries no ITIM or ITSM, and instead its cytoplasmic tyrosines Y256 and Y263 are phosphorylated to displace HLA-B associated transcript 3 and permit Fyn dependent inhibitory signaling, which is why TIM-3 behaves as a context dependent regulator rather than a simple brake. Germline loss-of-function HAVCR2 variants cause subcutaneous panniculitis-like T cell lymphoma with hemophagocytic syndrome, giving a genuine disease knockin rationale distinct from a null. Because clinical antibodies do not cross-react, humanization is the allele for pharmacology, while conditional nulls with Cd4-Cre or Lyz2-Cre separate lymphoid from myeloid contributions, and combining with Pdcd1 loss is usually required before checkpoint phenotypes become measurable.
A conventional Havcr2 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Havcr2 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Havcr2 (TIM-3) knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Havcr2 Knockout allele
A germline null is easy to make and easy to overinterpret. Delete the coding exons spanning the IgV domain through the transmembrane segment so no soluble ectodomain remains to bind galectin 9. On C57BL/6 the null is viable with only modest enhancement of Th1 responses and no spontaneous autoimmunity, so single-mutant characterization looks negative unless the animals are challenged. The null is the closest genetic match to human loss-of-function variants, but mice do not reproduce the panniculitis-like lymphoma spontaneously, and additional pressure, either an inflammatory trigger or a second lesion, is generally needed before hemophagocytic features appear; treat that as an open question rather than an expectation. Practical endpoints are inflammatory cytokines including IL-1 beta and IL-18, ferritin and soluble CD25, splenic macrophage activation, and tumor rejection in compound crosses with Pdcd1.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Havcr2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Havcr2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Havcr2 Knockout mouse models are available?
When Havcr2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Havcr2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Havcr2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Havcr2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Havcr2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.