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Hey1 Conditional Knockout mouse models
Belonging to the HESR family, Hey1 is the mouse ortholog of human HEY1 and encodes a basic helix-loop-helix Orange domain repressor that is a direct Rbpj-dependent Notch target; it homodimerizes and heterodimerizes with Hey2 and Hes1, binds class B and class C sites, and represses cardiogenic and chondrogenic programs including Gata4-dependent transcription, contributing to arterial versus venous endothelial specification and cardiac cushion development. In humans, the recurrent HEY1 to NCOA2 fusion defines mesenchymal chondrosarcoma, which makes a fusion knockin a distinct and highly informative allele class from simple loss: the fusion asks whether a chimeric transcriptional activator initiates cartilage-lineage tumors, whereas a conditional null asks whether endogenous repression is required for vascular and valvular patterning. Single Hey1 nulls are viable because Hey2 and Hes1 compensate, and double Hey1 and Hey2 animals show the cardiovascular lethality, so plan compound genetics and an endothelial or mesenchymal driver alongside the primary allele.
Tissue restricted knockout of Hey1 keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Hey1 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Hey1 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Hey1-Flox | CKO | CKO | DEVELOPING | CKO 262394 | Inquire |
| These mice carry loxP sites flanking exon1-4 of Hey1 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Hey1 gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Hey1 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Hey1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Hey1 Conditional Knockout mouse models are available?
When Hey1 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Hey1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Hey1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Hey1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Hey1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Related models and routes
Breed this line with ITL
Send the Hey1 Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.