Hkdc1 Knockout mouse models
Hkdc1 encodes the mouse ortholog of human HKDC1, the fifth hexokinase family member, which retains hexokinase domain architecture but exhibits low glucose-phosphorylating activity and is expressed in liver, gut and placenta rather than in classic glycolytic tissues. Human variants near HKDC1 associate with maternal glucose levels during pregnancy and with gestational glucose handling, and mouse work implicates the protein in hepatic glucose tolerance, lipid handling and association with mitochondria and lipid droplets, suggesting a regulatory rather than high-flux catalytic role. Because the physiology is pregnancy and liver centered, allele choice should match: a whole-body null clarifies gestational glucose phenotypes, a hepatocyte conditional null with Alb-Cre separates liver-autonomous effects from placental contributions, and humanized knockins permit interrogation of specific human coding and noncoding variants. Hk1 and Hk2 supply the bulk of hexokinase activity in most tissues, so avoid total activity assays as a readout. Center endpoints on pregnancy glucose tolerance, hepatic lipid quantification and tracer-based flux.
Whole body loss of Hkdc1 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Hkdc1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Hkdc1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Hkdc1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 220104 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Hkdc1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Hkdc1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Hkdc1 Knockout mouse models are available?
When Hkdc1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Hkdc1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Hkdc1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Hkdc1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Hkdc1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.