Hras Knockout mouse models
Hras, ortholog of human HRAS, encodes a small GTPase that cycles between GDP and GTP states under Sos1-family exchange factors and p120 and Nf1 GAPs, engaging Raf kinases, PI3K, and RalGDS when loaded; its distinguishing feature is the hypervariable carboxy-terminal region, which carries both farnesylation and dual palmitoylation and therefore traffics through the Golgi to cholesterol-rich plasma membrane microdomains rather than the disordered regions favored by Kras. Somatic G12V and Q61L substitutions occur in bladder and head and neck carcinoma, while germline G12S and G13D cause Costello syndrome, giving three distinct allele classes: a Lox-Stop-Lox oncogenic knockin for tumor initiation, an endogenous RASopathy point mutation for developmental and cardiac phenotypes, and humanization for isoform-selective inhibitor testing. Hras nulls are viable because Kras carries the essential load, so single-gene deletion is rarely informative, and compound Hras and Nras alleles combined with tissue-restricted Cre give interpretable results.
Hras null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Hras Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Hras knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Hras-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 191092 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Hras Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Hras allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Hras Knockout mouse models are available?
When Hras Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Hras knockout embryonic lethal in mice?
It depends on background and allele design. Some Hras germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Hras animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Hras?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.