Htt Knockin mouse models
Huntington disease work centers on Htt, the mouse ortholog of human HTT, encoding huntingtin, a 350 kDa HEAT-repeat scaffold that organizes vesicular and axonal transport along microtubules through HAP1 and the dynein/dynactin machinery, sustains BDNF transcription by sequestering REST in the cytoplasm, and supports selective autophagy cargo recognition and ciliogenesis. Pathology follows CAG repeat expansion in exon 1 beyond roughly 36 units, generating an elongated polyglutamine tract that drives aggregation, aberrant exon 1 splicing into a highly toxic fragment, and somatic repeat instability in striatal medium spiny neurons through MSH3 and mismatch repair activity. Because complete Htt loss is embryonic lethal near gastrulation, conditional floxed alleles are mandatory for adult loss-of-function and lowering-safety studies, whereas full-length or humanized exon 1 CAG knockins preserve endogenous regulation for instability and biomarker endpoints far better than randomly integrated transgenes. Align allele class with endpoint, pairing neuron-restricted or striatum-restricted Cre drivers with region-specific silencing questions.
Knockin designs at Htt support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Htt Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Htt knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Htt-hHTTQ145 | Knockin | point mutantion mice, Target humanized mice | sperm cryopreservation | KI 234514 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Htt Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Htt allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Htt Knockin mouse models are available?
When Htt Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Htt knockout embryonic lethal in mice?
It depends on background and allele design. Some Htt germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Htt animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Htt?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.