Idh1 Knockout mouse models
Cytosolic isocitrate dehydrogenase 1, encoded by Idh1 and orthologous to human IDH1, catalyzes the NADP-dependent oxidative decarboxylation of isocitrate to alpha-ketoglutarate in the cytoplasm and peroxisomes, supplying reducing equivalents for lipid synthesis and oxidative defense. Tumor alleles are heterozygous missense substitutions at arginine 132, most often R132H, which convert the enzyme into a neomorph that further reduces alpha-ketoglutarate to the oncometabolite D-2-hydroxyglutarate. Accumulated D-2HG competitively inhibits alpha-ketoglutarate dependent dioxygenases including TET2, the KDM4 and KDM6 demethylases, and prolyl hydroxylases, producing the CpG island hypermethylator phenotype and differentiation block characteristic of low grade glioma, chondrosarcoma, cholangiocarcinoma, and acute myeloid leukemia. Because the retained wild type allele is mechanistically required, the correct model is a conditional Lox-Stop-Lox R132H knockin at the endogenous locus rather than a null. Match Cre timing and lineage to the tumor type and use D-2HG suppression as the pharmacodynamic endpoint.
Idh1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Idh1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Idh1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Idh1-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 2110552 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Idh1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Idh1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Idh1 Knockout mouse models are available?
When Idh1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Idh1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Idh1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Idh1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Idh1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.