Idh2 Conditional Knockout mouse models
Idh2, the mouse ortholog of human IDH2, encodes the mitochondrial NADP-dependent isocitrate dehydrogenase that operates inside the matrix to generate alpha-ketoglutarate and NADPH for glutathione and thioredoxin regeneration, distinguishing it from the NAD-dependent heterotrimeric IDH3 that carries flux through the tricarboxylic acid cycle. Cancer alleles are heterozygous substitutions at arginine 140, most often R140Q in acute myeloid leukemia, and at arginine 172 in leukemia and glioma, each producing the same neomorphic reduction of alpha-ketoglutarate to D-2-hydroxyglutarate that inhibits TET2, the JmjC demethylases, and collagen prolyl hydroxylases, driving DNA and histone hypermethylation with a block in myeloid differentiation. Conditional Lox-Stop-Lox knockin of the specific residue, not deletion, reproduces this biology, and the choice between R140Q and R172K matters because they differ in enzymatic output and inhibitor sensitivity. Use an inducible hematopoietic Cre, and pair with Flt3 or Npm1 alleles when full transformation is the endpoint.
Tissue restricted knockout of Idh2 keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Idh2 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Idh2 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Idh2-Flox | Conditional Knockout | KO/CKO mice, disease model mice | live | CKO 205094 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Idh2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Idh2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Idh2 Conditional Knockout mouse models are available?
When Idh2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Idh2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Idh2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Idh2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Idh2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.