Ifng Knockout mouse models
Interferon gamma, encoded by Ifng, is the mouse ortholog of human IFNG and the only type II interferon, a homodimeric cytokine that clusters IFNGR1 with IFNGR2, activates JAK1 and JAK2, and drives STAT1 homodimers onto gamma activated sequences to induce CIITA and MHC class II, immunoproteasome subunits, CXCL9 and CXCL10, and nitric oxide synthase in primed macrophages. Its sources are Th1 cells, NK cells, ILC1s, and cytotoxic lymphocytes, and human loss-of-function lesions in this pathway cause Mendelian susceptibility to mycobacterial disease, whereas excess drives hemophagocytic pathology. Allele choices diverge sharply: a conditional null asks whether a given lymphocyte lineage supplies the cytokine, a knockin reporter reads transcription without perturbing it, and humanization supports emapalumab-class neutralization studies. Pair the Ifng flox with Cd4-Cre, Cd8-Cre, or Ncr1-Cre rather than germline deletion when the goal is attributing the cellular source rather than ablating the axis entirely.
A conventional Ifng knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ifng Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Ifng knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ifng Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ifng allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ifng Knockout mouse models are available?
When Ifng Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ifng knockout embryonic lethal in mice?
It depends on background and allele design. Some Ifng germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ifng animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ifng?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.