Ikbke Knockout mouse models
Ikbke encodes IKK epsilon, the mouse ortholog of human IKBKE and the noncanonical IKK family kinase most closely related to TBK1, sharing a kinase domain, ubiquitin-like domain, and scaffold dimerization region. Together with TBK1 it phosphorylates IRF3 on the C-terminal serine cluster to license interferon beta transcription, and it also phosphorylates STAT1 at Ser708 to reshape ISGF3 output and modifies NF-kB regulators including CYLD, which underlies its behavior as an amplified breast cancer oncogene able to substitute for AKT driven survival signaling. Design follows the question directly: a conditional null removes protein and scaffolding together, whereas a kinase-dead knockin distinguishes catalytic activity from complex assembly, the decisive comparison for evaluating small molecule inhibitors. Substantial redundancy with Tbk1 means single deletion blunts rather than abolishes interferon induction, and because Tbk1 nulls die in utero from TNF driven hepatocyte apoptosis, compound experiments require conditional Tbk1 alleles planned in advance.
A conventional Ikbke knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ikbke Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Ikbke knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ikbke Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ikbke allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ikbke Knockout mouse models are available?
When Ikbke Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ikbke knockout embryonic lethal in mice?
It depends on background and allele design. Some Ikbke germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ikbke animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ikbke?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.