Il12a Conditional Knockout mouse models
Il12a, the mouse ortholog of human IL12A, encodes the p35 subunit, and its central design trap is subunit sharing: p35 heterodimerizes with p40 from Il12b to form bioactive interleukin 12, and with EBI3 to form interleukin 35, an inhibitory cytokine of regulatory T and B cells. Interleukin 12 acts through IL-12Rbeta1 and IL-12Rbeta2 to trigger JAK2 and STAT4, imprinting Th1 commitment and interferon gamma production, while interleukin 35 signals through gp130 with IL-12Rbeta2 to activate STAT1 and STAT4 in a suppressive configuration. A germline Il12a null therefore removes a proinflammatory and an anti-inflammatory cytokine simultaneously, so phenotypes read as net effects rather than clean interleukin 12 loss. Dissection requires triangulation across Il12b, Ebi3, and Il12rb2 alleles, plus conditional Il12a deletion with dendritic cell or B cell restricted Cre drivers, and humanization when the endpoint is ustekinumab-class or p35 directed antibody pharmacology.
Tissue restricted knockout of Il12a keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Il12a Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Il12a conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Il12a-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 2108334 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Il12a Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Il12a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Il12a Conditional Knockout mouse models are available?
When Il12a Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Il12a knockout embryonic lethal in mice?
It depends on background and allele design. Some Il12a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Il12a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Il12a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.