Il12b Knockin mouse models
The p40 subunit encoded by Il12b, mouse ortholog of human IL12B, is the shared chain of two distinct heterodimeric cytokines, interleukin 12 when paired with p35 and interleukin 23 when paired with p19. Interleukin 12 drives STAT4 dependent Th1 differentiation and interferon gamma, whereas interleukin 23 sustains RORgt dependent Th17 and ILC3 programs with interleukin 17 and interleukin 22 output, so a single Il12b null collapses both axes at once and cannot distinguish them. This is precisely the ambiguity that made p40 blockade with ustekinumab clinically effective yet mechanistically opaque, and why selective p19 blockade later outperformed it in psoriasis. Rational design pairs Il12b alleles with Il12a and Il23a single nulls to partition the two cytokines, uses conditional deletion in dendritic cells or macrophages to identify the producing compartment, and adopts humanization when p40 antibody pharmacology rather than pathway ablation is the endpoint. Homodimeric p40 can also antagonize receptor engagement, complicating partial loss interpretation.
An engineered Il12b allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Il12b Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Il12b knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Il12b-Luc-2A-EGFP | Knockin | fluorescent mouse | live | KI 00025 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Il12b Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Il12b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Il12b Knockin mouse models are available?
When Il12b Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Il12b knockout embryonic lethal in mice?
It depends on background and allele design. Some Il12b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Il12b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Il12b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.