Il27 Knockout mouse models
Il27 encodes the p28 subunit, mouse ortholog of human IL27, which pairs with EBI3 to form interleukin 27 and signals through IL-27Ralpha, also called WSX-1, together with gp130 to activate both STAT1 and STAT3. That dual output explains its split personality, with early amplification of Th1 commitment and T-bet alongside potent suppression of Th17 differentiation, induction of interleukin 10 producing Tr1 cells, and upregulation of inhibitory receptors including PD-L1 and TIM-3 on T cells. Two design subtleties matter. EBI3 is shared with interleukin 35, so an Ebi3 null removes two cytokines while an Il27 null removes only p28 containing species, and unpaired p28 can antagonize gp130 signaling, meaning overexpression and deletion are not simple mirror images. Conditional deletion in dendritic cells or macrophages assigns the source, Il27ra alleles test receptor requirement in T cells, and humanization supports antibody pharmacology where the immunosuppressive arm is the therapeutic target.
Il27 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Il27 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Il27 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Il27 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Il27 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Il27 Knockout mouse models are available?
When Il27 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Il27 knockout embryonic lethal in mice?
It depends on background and allele design. Some Il27 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Il27 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Il27?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.