Il5 Knockout mouse models
Encoding a homodimeric type 2 cytokine, Il5 corresponds to human IL5 and signals through IL-5Ralpha paired with the beta common chain encoded by Csf2rb, shared with interleukin 3 and GM-CSF, activating JAK2 and STAT5 to control eosinophil lineage commitment, marrow release, tissue survival, and B1 cell antibody production. It is the dominant nonredundant eosinophil axis, which is why mepolizumab and reslizumab neutralize the ligand while benralizumab depletes eosinophils by targeting the receptor alpha chain with an afucosylated Fc. The locus sits in the type 2 cluster near Il4 and Il13 under shared regulatory elements, so verify that any targeting cassette does not alter neighboring transcription. Design accordingly: conditional deletion with Cd4-Cre or ILC2 restricted drivers attributes the source, receptor alleles separate development from survival, and humanization supports ligand and receptor antibody pharmacology. Avoid Csf2rb deletion as a proxy, since it also removes interleukin 3 and GM-CSF signaling.
A conventional Il5 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Il5 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Il5 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Il5-KO | Knockout | KO/CKO mice | live | KO 190505 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Il5 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Il5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Il5 Knockout mouse models are available?
When Il5 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Il5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Il5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Il5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Il5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.