Insr Knockout mouse models
Insr, the mouse ortholog of human INSR, encodes a disulfide-linked receptor tyrosine kinase assembled as an alpha-beta heterotetramer, with L1, L2, and cysteine-rich ligand-binding modules and a kinase domain whose activation loop tyrosines undergo trans-autophosphorylation on insulin binding, creating NPXY docking sites for Irs1 and Irs2 that route signals into PI3K, Akt, and Foxo1 as well as Shc-dependent Erk activation; exon 11 splicing generates IR-A and IR-B isoforms with different affinities and tissue distributions. Homozygous nulls are born grossly normal but die within days from ketoacidosis, mirroring human Donohue syndrome, so tissue-restricted deletion with Alb-Cre, Ckmm-Cre, Adipoq-Cre, or Ins1-Cre is the standard approach for dissecting organ-specific insulin action. Kinase-dead and NPXY-mutant knockins separate catalysis from scaffolding, and humanization supports analog and bispecific antibody testing. Igf1r is a close paralog that forms hybrid receptors and buffers signaling, so compound alleles are often necessary.
Insr null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Insr Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Insr knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Insr-KO | Knockout | KO/CKO mice, disease model mice | embryo cryopreservation | KO 205049 | Inquire |
Designing a Insr Knockout allele
Homozygous nulls survive birth and then die within days from unrestrained lipolysis, hyperglycemia and ketoacidosis, so the allele supports only a narrow neonatal window. That window is still useful. Birth weight is reduced by roughly ten percent, which quantifies the contribution of Igf2 signaling through the A isoform to fetal growth, and postnatal day one to three tissue is enough for hepatic glycogen, lipolysis markers and receptor-proximal signaling. The more productive use is dosage genetics: heterozygotes are only mildly insulin resistant alone but become overtly diabetic when combined with Irs1 heterozygosity, which makes this allele a defined sensitizer for modifier screens. A hypomorphic design that lowers expression rather than eliminating it is the alternative when adult survival is required. Track survival curves, glucose and ketones from the first hours, birth weight, hepatic glycogen depletion, and insulin-stimulated phospho-Akt in explanted tissue.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Insr Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Insr allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Insr Knockout mouse models are available?
When Insr Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Insr knockout embryonic lethal in mice?
It depends on background and allele design. Some Insr germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Insr animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Insr?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.