Irf7 Knockout mouse models
Serving as the master amplifier of the type I interferon system, Irf7 is the mouse ortholog of human IRF7 and encodes an interferon inducible regulatory factor that closes the positive feedback loop converting the single Ifnb1 first wave into broad interferon alpha subtype expression. In plasmacytoid dendritic cells it is activated MyD88 dependently through IRAK4, IRAK1, and IKKalpha downstream of endosomal TLR7 and TLR9, and in other cells by TBK1, with phosphorylation of the C-terminal serine cluster licensing dimerization and ISRE occupancy. Human IRF7 deficiency causes life threatening influenza with preserved responses to many other pathogens, while elevated interferon tone is central to lupus biology. Because Irf3 initiates and Irf7 amplifies, a single null blunts kinetics rather than abolishing induction, so compound alleles are frequently required. Conditional deletion with plasmacytoid dendritic cell restricted or Itgax-Cre drivers assigns the amplification compartment, and reporter knockins time the feedback loop without perturbing it.
A conventional Irf7 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Irf7 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Irf7 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Irf7 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Irf7 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Irf7 Knockout mouse models are available?
When Irf7 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Irf7 knockout embryonic lethal in mice?
It depends on background and allele design. Some Irf7 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Irf7 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Irf7?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.