Irf3 Knockout mouse models
Irf3 corresponds to human IRF3 and encodes a constitutively expressed interferon regulatory factor with an N-terminal winged helix DNA binding domain and a C-terminal IRF association domain held autoinhibited until TBK1 or IKK epsilon phosphorylate the serine-rich tail at Ser386 and Ser396 downstream of STING, MAVS, or TRIF. Phosphorylation drives dimerization, nuclear accumulation, CBP and p300 recruitment, and transcription of Ifnb1 with early interferon stimulated genes, while a transcription independent arm, the RLR induced apoptotic pathway, uses BH3 like engagement of BAX to kill infected cells. Allele forks map cleanly onto these functions: conditional nulls test requirement, phosphomimetic constitutively active knockins model chronic interferon tone, and mutations separating transactivation from BAX binding isolate the apoptotic arm. Irf7 substantially overlaps in interferon induction, so compound Irf3 with Irf7 deletion is usually required before output is truly abolished, and STING gain-of-function crosses need conditional alleles to survive breeding.
A conventional Irf3 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Irf3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Irf3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Irf3-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 201668 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Irf3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Irf3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Irf3 Knockout mouse models are available?
When Irf3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Irf3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Irf3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Irf3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Irf3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.