Jak2 Knockin mouse models
Encoding the kinase that pairs with single-chain cytokine receptors, Jak2 is the mouse ortholog of human JAK2 and transduces signals from Epor, Mpl, Ghr, Prlr, and Ifngr, phosphorylating Stat5 in particular; its pseudokinase JH2 domain normally restrains the catalytic domain, and the Val617Phe substitution in that interface produces constitutive, ligand-independent activation that drives polycythemia vera, essential thrombocythemia, and primary myelofibrosis, with exon 12 insertions and deletions giving erythroid-restricted disease. Homozygous nulls die at mid-gestation from absent definitive erythropoiesis, so conditional deletion is mandatory, and the informative activating design is a conditional Val617Phe knockin at the endogenous locus expressed through Vav-Cre or interferon-inducible Mx1-Cre, which reproduces allele-dosage-dependent myeloproliferative phenotypes far more faithfully than transgenic overexpression. Humanization supports type I and type II JAK2 inhibitor pharmacology. Receptor pairing is largely nonredundant, so Jak1 and Tyk2 cannot rescue erythropoiesis; match allele class, dosage, and driver to the hematopoietic endpoint.
An engineered Jak2 allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Jak2 Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Jak2 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Jak2 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Jak2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Jak2 Knockin mouse models are available?
When Jak2 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Jak2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Jak2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Jak2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Jak2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.