Kcnj10 Knockout mouse models
Kcnj10, the mouse ortholog of human KCNJ10, encodes Kir4.1, the principal inwardly rectifying potassium channel of astrocytes, Bergmann glia, oligodendrocytes, and retinal Muller cells, where it clamps the glial membrane near the potassium equilibrium potential and performs spatial buffering of extracellular potassium released during neuronal firing. Kir4.1 functions as a homotetramer or as an obligate partner in Kir4.1/Kir5.1 heteromers with the Kcnj16 product, which confers steep intracellular pH sensitivity, and it also operates in the distal convoluted tubule and stria vascularis, coupling to the sodium pump and to chloride handling. Biallelic human mutations cause SeSAME/EAST syndrome with seizures, ataxia, sensorineural deafness, and renal salt wasting, and constitutive nulls die in the early postnatal period with hypomyelination and severe motor impairment, so glia-restricted conditional deletion using Gfap-Cre or Aldh1l1-CreER is required for adult work. No other glial Kir substitutes effectively, so match allele to whether the endpoint is potassium clearance, seizure threshold, or renal electrolyte handling.
Global Kcnj10 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Kcnj10 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Kcnj10 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kcnj10-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 200281 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Kcnj10 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kcnj10 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kcnj10 Knockout mouse models are available?
When Kcnj10 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kcnj10 knockout embryonic lethal in mice?
It depends on background and allele design. Some Kcnj10 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kcnj10 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kcnj10?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.