Kcnj13 Knockin mouse models
Kcnj13, the mouse ortholog of human KCNJ13, encodes Kir7.1, an atypical inward rectifier with unusually low single-channel conductance and weak dependence on extracellular potassium, properties traced to a methionine at the position occupied by arginine in other Kir channels. Kir7.1 is concentrated at the apical membrane of retinal pigment epithelium, where it colocalizes with the sodium pump and sets subretinal potassium levels, and it is also expressed in choroid plexus, thyroid, and uterine smooth muscle. Human mutations cause snowflake vitreoretinal degeneration through dominant-negative gating changes and Leber congenital amaurosis type 16 through recessive loss of function, giving a clear allele fork between knockin and null. Constitutive mouse nulls die within a day of birth with cleft palate and feeding failure, so retinal pigment epithelium-restricted conditional deletion using Best1-Cre or an inducible driver is required to study photoreceptor survival and electroretinogram c-wave endpoints in adults, and humanized alleles support gene replacement testing.
An engineered Kcnj13 allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Kcnj13 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Kcnj13 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kcnj13-CreERT2 | Knockin | Cre/Dre Toolbox of Mice | live | KI 241142 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Kcnj13 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kcnj13 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kcnj13 Knockin mouse models are available?
When Kcnj13 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kcnj13 knockout embryonic lethal in mice?
It depends on background and allele design. Some Kcnj13 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kcnj13 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kcnj13?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.