Kcnj16 Knockout mouse models
Kcnj16, the mouse ortholog of human KCNJ16, encodes Kir5.1, a subunit that is electrically silent on its own and functions only by heteromerizing with Kir4.1 or Kir4.2, where it converts a weakly pH-sensitive channel into a steeply pH-sensitive one through a lysine in the second transmembrane domain of its partner and through a Kir5.1-specific carboxy-terminal interface. Kir4.1/Kir5.1 channels operate on the basolateral membrane of the distal convoluted tubule and collecting duct, in astrocytes, in carotid body and brainstem chemosensitive neurons, and in retina, making the subunit a molecular link between carbon dioxide, intracellular pH, and potassium conductance. Null rats and mice show impaired potassium and acid-base handling with hypokalemic alkalosis and altered chemoreflex responses. Because the obligate partnership is with Kcnj10 and Kcnj15 products, deletion of Kcnj16 does not abolish current but shifts pH sensitivity, so design endpoints around gating and transport physiology rather than total conductance, using nephron or glia-restricted Cre.
Global Kcnj16 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Kcnj16 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Kcnj16 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kcnj16-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 200285 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Kcnj16 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kcnj16 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kcnj16 Knockout mouse models are available?
When Kcnj16 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kcnj16 knockout embryonic lethal in mice?
It depends on background and allele design. Some Kcnj16 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kcnj16 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kcnj16?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.