Kcnj4 Knockout mouse models
Kcnj4, the mouse ortholog of human KCNJ4, encodes Kir2.3, a strong inward rectifier of the Kir2 family that differs from Kir2.1 and Kir2.2 in having lower single-channel conductance, weaker PIP2 affinity, and pronounced inhibition by intracellular protons, making it the pH-sensitive member of the group. It is enriched in forebrain neurons, particularly in hippocampus and cortex where it contributes to resting conductance and dendritic input resistance, and it is present in atrial myocytes and kidney. Its carboxy-terminal PDZ-binding motif recruits PSD-95 and SAP97, localizing the channel to synaptic and lateral membrane domains. Because Kir2.3 heteromerizes freely with Kir2.1 and Kir2.2 encoded by Kcnj2 and Kcnj12, single-locus nulls are viable and usually show only partial IK1 reduction, so compound conditional alleles or dominant-negative pore knockins are required to unmask function. Align the allele with the endpoint, using forebrain-restricted Cre for excitability studies and pH-sensing residue knockins for gating mechanism.
Kcnj4 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Kcnj4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Kcnj4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kcnj4-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 200287 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Kcnj4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kcnj4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kcnj4 Knockout mouse models are available?
When Kcnj4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kcnj4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Kcnj4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kcnj4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kcnj4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.