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Kit Xenograft-Applicable mouse models
Kit encodes the mouse ortholog of human KIT, a type III receptor tyrosine kinase with five extracellular immunoglobulin-like domains, an autoinhibitory juxtamembrane segment, and a split kinase domain that dimerizes upon binding stem cell factor (Kitl). Ligand engagement triggers trans-autophosphorylation and recruitment of PI3K, SRC family kinases, and the RAS-ERK cascade, sustaining mast cells, melanoblasts, hematopoietic progenitors, germ cells, and interstitial cells of Cajal. Oncogenic alleles fall into two mechanistic classes: juxtamembrane exon 11 deletions that relieve autoinhibition in gastrointestinal stromal tumor, and activation loop D816V substitutions that confer imatinib resistance in systemic mastocytosis and core binding factor leukemia. Conditional nulls therefore address lineage dependency, whereas knockin of the exact human hotspot codon models spontaneous tumorigenesis and inhibitor pharmacology; complete loss recapitulates the dominant white spotting phenotype with perinatal macrocytic anemia, so hypomorphic or inducible strategies preserve viability. Pair activating knockins with tissue-restricted Cre drivers to localize transformation and obtain clean drug response endpoints.
Kit engineered hosts can be tuned for xenograft take rate and growth kinetics depending on tumor lineage.
What Kit Xenograft-Applicable mouse models are available?
ingenious targeting laboratory offers 1 distinct Kit xenograft-applicable catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kit-V831M/hIL3-hCSF2-Tg(M-NSG) | XA | Point Mutation, Target-Humanized, Immunodeficient, Random Transgenic | LIVE | XA 261535 | Inquire |
| The Kit-V831M(NMG) (KI 242813) was crossed with hIL3-hCSF2-Tg(NMG)(KI 215006) to generate Kit-V831M(NMG)/hIL3-hCSF2-Tg(NMG) mice. | |||||
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Kit Xenograft-Applicable lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kit allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kit Xenograft-Applicable mouse models are available?
When Kit Xenograft-Applicable lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kit knockout embryonic lethal in mice?
It depends on background and allele design. Some Kit germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kit animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kit?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Related models and routes
Citations
- Loss-of-function OGFRL1 variants identified in autosomal recessive cherubism families
- Thrombin-activated interleukin-1α drives atherogenesis, but also promotes vascular smooth muscle cell proliferation and collagen production.
- Alveolar bone protection by targeting the SH3BP2-SYK axis in osteoclasts
- The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin
- Cell type-specific genetic and optogenetic tools reveal hippocampal CA2 circuits.
Breed this line with ITL
Send the Kit Xenograft-Applicable line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.