Kras Xenograft-Applicable mouse models
Kras, the mouse ortholog of human KRAS, encodes the Ras isoform on which most epithelial tumors depend, a GTPase governed by Sos1-mediated exchange and by Nf1 and Rasa1 GAP activity, coupling receptor input to Raf, PI3K, and Ral effectors; unlike Hras it is farnesylated without palmitoylation and carries a polybasic hypervariable region, and alternative fourth-exon splicing produces the 4A and 4B isoforms with different membrane behavior. Codon 12, 13, and 61 substitutions abolish GAP-stimulated hydrolysis, and allele identity matters pharmacologically, since Gly12Cys and Gly12Asp are addressed by chemically distinct inhibitors. Homozygous deletion is embryonic lethal, uniquely among Ras genes, so conditional nulls are required, while Lox-Stop-Lox oncogenic knockins expressed at endogenous levels remain the standard for tumor initiation and humanization enables clinical-compound pharmacokinetic and pharmacodynamic testing. Hras and Nras can substitute developmentally when knocked into the Kras locus, evidence that dosage and localization, not sequence identity, drive the essential requirement.
Orthotopic and subcutaneous routes both benefit from consistent Kras genetics across cages.
What Kras Xenograft-Applicable mouse models are available?
ingenious targeting laboratory offers 3 distinct Kras xenograft-applicable catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kras-LSL-G12D/Pdx1-Cre-Tg/Tgfbr2-Flox | Xenograft-Applicable | KO/CKO mice,point mutantion mice,Cre/Dre Toolbox of Mice, disease model mice, random transgenic mice | embryo cryopreservation | XA 234935 | Inquire |
| Kras-LSL-G12D/Trp53-LSL-R172H | Xenograft-Applicable | point mutantion mice | live | XA 253119 | Inquire |
| Kras-LSL-G12D/Trp53-LSL-R172H/Pdx1-2A-Cre | Xenograft-Applicable | Cre/Dre Toolbox of Mice | live | XA 242420 | Inquire |
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Kras Xenograft-Applicable lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kras allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kras Xenograft-Applicable mouse models are available?
When Kras Xenograft-Applicable lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kras knockout embryonic lethal in mice?
It depends on background and allele design. Some Kras germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kras animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kras?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.