Ldlr Conditional Knockout mouse models
Ldlr, the mouse ortholog of human LDLR, encodes the cell surface receptor that clears apoB-containing lipoproteins, built from seven class A cysteine-rich ligand-binding repeats, EGF-A and EGF-B modules flanking a beta-propeller that mediates acid-dependent ligand release, an O-linked sugar domain, and a cytoplasmic NPXY motif read by Ldlrap1 for clathrin-mediated internalization; Pcsk9 binding to EGF-A diverts the receptor to lysosomal degradation instead of recycling, and Mylip ubiquitination provides a sterol-responsive degradation route. Loss-of-function alleles cause familial hypercholesterolemia, and null mice are viable but develop hypercholesterolemia and atherosclerosis on Western diet, making them a standard cardiovascular platform. Allele choice follows the question: a null for plaque biology, patient point mutations for class-specific trafficking defects, and full humanization when the endpoint is PCSK9 antibody efficacy, siRNA silencing, or base editing of the human sequence. Combining with Apoe or Apobec1 alleles tunes lipoprotein profiles toward human-like distributions.
Conditional deletion of Ldlr limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Ldlr floxed stock yields usable cohorts.
What Ldlr Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ldlr conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ldlr-Flox | Conditional Knockout | KO/CKO mice | live | CKO 210188 | Inquire |
Designing a Ldlr Conditional Knockout allele
A germline Ldlr null survives and produces the desired hypercholesterolemia, so a floxed allele is not a rescue from lethality but a tool for tissue attribution: hepatocytes handle most apoB particle clearance, and everything else, adrenal cortex, ovary, macrophage, endothelium, is normally invisible behind that hepatic signal. Flank the exons encoding ligand-binding repeats one through five so that the transcript cannot produce a receptor with residual apoB affinity, rather than trimming the cytoplasmic tail, which leaves a surface-retained binding-competent protein. Alb-Cre or AAV8-TBG-Cre gives hepatocyte-restricted loss, LysM-Cre addresses lesional macrophage uptake, and Cdh5-CreERT2 tests endothelial transcytosis. Watch mosaicism: incomplete hepatic recombination leaves enough receptor to blunt the plasma cholesterol rise, so quantify by DiI-LDL uptake in isolated hepatocytes and surface receptor flow cytometry, not genotype alone. Read out FPLC lipoprotein distribution alongside aortic root lesion area.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Ldlr Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ldlr allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ldlr Conditional Knockout mouse models are available?
When Ldlr Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ldlr knockout embryonic lethal in mice?
It depends on background and allele design. Some Ldlr germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ldlr animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ldlr?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.