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Lefty2 Conditional Knockout mouse models
Serving as the diffusible inhibitor arm of the left-right circuit, Lefty2 is the mouse ortholog of human LEFTY2 and encodes a secreted, cleaved TGF-beta family protein that captures Nodal and the EGF-CFC coreceptors Cripto and Cryptic, preventing ActRIIB and ALK4 engagement and limiting SMAD2 and SMAD3 output. Because Nodal induces Lefty2 in left lateral plate mesoderm, the pair constitutes a self-regulating activator with a long-range inhibitor that positions the Pitx2 domain and fixes situs. Loss causes embryonic lethality from excess Nodal signaling with expanded mesendoderm, and human variants appear in heterotaxy and complex cardiac malformation. Consequently, conditional and hypomorphic alleles are preferable to germline nulls for laterality work, deletion of the asymmetric enhancer separates Nodal-dependent induction from constitutive expression, and dosage-sensitive knockins probe inhibitor range. Lefty1 is tandemly linked and functionally overlapping, so compound alleles require cis engineering rather than conventional intercrossing.
Lefty2 conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype.
What Lefty2 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Lefty2 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Lefty2-Flox | CKO | CKO | IN PRODUCTION | CKO 254075 | Inquire |
| These mice carry loxP sites flanking exon 4 of Lefty2 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Lefty2 gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Lefty2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Lefty2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Lefty2 Conditional Knockout mouse models are available?
When Lefty2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Lefty2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Lefty2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Lefty2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Lefty2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the Lefty2 Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.