Lepr Knockout mouse models
Lepr, ortholog of human LEPR, encodes the leptin receptor, a class I cytokine receptor expressed as several splice isoforms of which only the long form carries the full intracellular Box1 and Box2 motifs needed to activate Jak2 and the downstream tyrosines that recruit SHP2 and ERK, STAT5, and STAT3 to drive Pomc and Agrp transcription in hypothalamic neurons. This isoform structure is the crux of allele design, since the classic db mutation is a splicing defect that eliminates the signaling isoform while leaving short forms intact, and single tyrosine knockins that block STAT3 recruitment reproduce obesity without the full null phenotype, cleanly separating individual signaling arms. Conditional deletion with neuron-subtype Cre drivers assigns energy balance and glucose control to defined populations, and Lepr-Cre is itself a knockin requiring dosage controls. Match alleles to endpoints spanning body composition, food intake, hepatic glucose production, and phosphorylated STAT3 immunostaining.
Global Lepr deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Lepr Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Lepr knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Lepr-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 190663 | Inquire |
Designing a Lepr Knockout allele
An engineered whole-locus null is not equivalent to the classic db allele, and the difference matters. The db mutation is a splice defect that abolishes only the signaling isoform, leaving short receptor forms available for leptin transport and clearance, whereas deleting the locus removes those too, which raises circulating leptin further and eliminates any short-form-dependent function. Choose accordingly: use the null when the question is whether a ligand or analog acts through this receptor at all, and use db when comparability with the historical literature is the priority. Background dominates the phenotype, with frank beta-cell failure and diabetes on C57BLKS versus compensated hyperinsulinemic obesity on C57BL/6, so report the strain explicitly. Expect infertility, reduced linear growth and immune changes alongside obesity. Endpoints include weight trajectory, hyperphagia, fasting glucose and insulin, glucose tolerance, islet morphometry, and absence of arcuate phospho-STAT3 after leptin injection.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Lepr Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Lepr allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Lepr Knockout mouse models are available?
When Lepr Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Lepr knockout embryonic lethal in mice?
It depends on background and allele design. Some Lepr germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Lepr animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Lepr?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.