Mapk14 Conditional Knockout mouse models
Mapk14, the mouse ortholog of human MAPK14, encodes p38alpha, a stress-activated MAP kinase phosphorylated on the Thr and Tyr residues of its TGY motif by Map2k3 and Map2k6 downstream of Tak1, Ask1, and Map3k7 in response to inflammatory cytokines, osmotic stress, and DNA damage; its substrates include Mapkapk2, Mknk1, Atf2, Mef2c, and Tp53, and it governs Tnf and Il1b mRNA stability through the MK2 and tristetraprolin axis. A Thr106 gatekeeper residue defines the ATP pocket exploited by pyridinylimidazole inhibitors, which is why a Thr106Met knockin creating a drug-resistant kinase remains the most rigorous in vivo test of on-target inhibitor action. Homozygous nulls die at mid-gestation from placental defects that tetraploid complementation rescues, so conditional deletion is standard for immune and cardiac studies. Mapk11, Mapk12, and Mapk13 encode the other p38 isoforms with partially shared substrates, so compound alleles and a validated lineage driver protect against masked phenotypes.
Conditional deletion of Mapk14 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Mapk14 floxed stock yields usable cohorts.
What Mapk14 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Mapk14 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Mapk14-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 2102209 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Mapk14 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mapk14 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mapk14 Conditional Knockout mouse models are available?
When Mapk14 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mapk14 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mapk14 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mapk14 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mapk14?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.