Mapt Knockin mouse models
Mapt, the mouse ortholog of human MAPT, encodes tau, a natively unfolded microtubule-associated protein whose carboxy-terminal repeat domain binds along the microtubule lattice to promote assembly and regulate axonal transport, while its projection domain and extensive phosphorylation by GSK3 beta, CDK5, and MARK kinases control detachment and aggregation propensity. A decisive species difference drives model design: adult mouse brain expresses almost exclusively four-repeat tau, whereas human brain expresses balanced three-repeat and four-repeat isoforms generated by exon 10 splicing, so intronic and exonic FTDP-17 mutations that shift isoform ratio cannot be modeled on the mouse locus and require humanized MAPT knockin alleles carrying the full genomic region. Coding mutations such as P301S and P301L instead drive filament formation and are commonly modeled by knockin or transgene. Nulls are viable with mild phenotypes because Map1b and Map2 compensate, so deletion answers requirement while humanized or aggregation-prone knockins answer tauopathy mechanism and antibody or antisense oligonucleotide response.
Knockin designs at Mapt support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Mapt Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Mapt knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Mapt-R5H | Knockin | point mutantion mice | sperm cryopreservation | KI 215099 | Inquire |
Designing a Mapt Knockin allele
State the limitation first. Adult mouse brain makes almost exclusively four-repeat tau and murine tau aggregates poorly, so a coding mutation placed in the mouse locus yields weaker pathology than the same change in human sequence. P301 lies within the first microtubule-binding repeat encoded by the alternatively spliced exon, so a mouse-locus knockin does put the substitution in correct structural context, but expect long latency and low filament burden at endogenous dosage. Intronic and exonic splice-shifting FTDP-17 mutations cannot be modeled here at all, because the isoform ratio they perturb does not exist in adult mouse brain. If filament formation and antibody or oligonucleotide response are the goal, place the mutation in a humanized allele instead. Endpoints: sarkosyl-insoluble tau, conformational and phospho-epitope staining, Gallyas silver, and seeding activity in a biosensor assay.
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Mapt Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mapt allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mapt Knockin mouse models are available?
When Mapt Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mapt knockout embryonic lethal in mice?
It depends on background and allele design. Some Mapt germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mapt animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mapt?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.