Mlh1 Knockout mouse models
Mlh1, the mouse ortholog of human MLH1, encodes the obligate partner of the MutL family, dimerizing with Pms2 to form MutLalpha, and less commonly with Mlh3 or Pms1; recruited by mismatch-bound MutSalpha, the complex uses ATP binding and the latent Pms2 endonuclease to nick the nascent strand, licensing Exo1-mediated excision and resynthesis, and it separately cooperates with Mlh3 to resolve meiotic recombination intermediates into crossovers. Germline loss causes Lynch syndrome with microsatellite instability, and somatic promoter hypermethylation silences it in sporadic colorectal and endometrial cancer. Homozygous nulls are viable but cancer-prone and sterile from meiotic failure, so conditional alleles are the way to study tissue-restricted repair loss without confounding infertility. Endonuclease-dead and dimerization-selective knockins separate repair from crossover functions, while humanization enables functional classification of variants of uncertain significance. Mlh3 and Pms1 partially cover meiotic roles but cannot substitute for MutLalpha in repair, so compensation is limited.
Mlh1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Mlh1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Mlh1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Mlh1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mlh1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mlh1 Knockout mouse models are available?
When Mlh1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mlh1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mlh1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mlh1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mlh1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.