Mmp2 Knockout mouse models
Mmp2 is the mouse ortholog of human MMP2 and encodes gelatinase A, distinguished from most family members by three fibronectin type II repeats inserted in the catalytic domain that confer high-affinity binding to gelatin, collagen, and elastin, and by an activation route requiring cell surface MMP14 with TIMP2 acting as a bridging cofactor rather than soluble proteolysis. Principal substrates include type IV collagen of basement membranes plus laminin, fibronectin, and latent TGF beta binding proteins, placing it centrally in angiogenic sprouting, tumor cell intravasation, and cardiac remodeling. Human biallelic MMP2 mutations cause multicentric osteolysis nodulosis and arthropathy, giving a defined disease knockin option, whereas mouse nulls are viable with modest growth retardation and reduced neovascularization. Overlapping gelatinolysis by Mmp9 means single deletion often looks mild, so plan compound alleles when basement membrane integrity is the readout, and use endothelial or fibroblast restricted Cre lines for source assignment.
A conventional Mmp2 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Mmp2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Mmp2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Mmp2-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 201725 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Mmp2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mmp2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mmp2 Knockout mouse models are available?
When Mmp2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mmp2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mmp2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mmp2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mmp2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.