Mmp13 Knockout mouse models
Encoding collagenase 3, Mmp13 is the mouse ortholog of human MMP13 and the principal rodent interstitial collagenase, secreted as a zymogen activated by MMP2, MMP14, or plasmin and inhibited by TIMPs, with a catalytic domain that cleaves type I, II, and III collagen and a hemopexin domain that also permits degradation of aggrecan fragments, fibronectin, and perlecan. Transcription is driven by RUNX2, interleukin 1 beta, and MAPK signaling, and the enzyme is the dominant proteolytic driver of osteoarthritic cartilage loss as well as a required participant in growth plate remodeling and endochondral ossification, where compound loss with Mmp9 produces severe skeletal phenotypes. Design should therefore favor conditional deletion, using Col2a1-CreER or Acan-CreER for cartilage and osteoblast drivers for bone, rather than germline nulls that confound development with disease. Catalytically inactive knockins separate proteolysis from other functions, and humanization supports selective inhibitor testing where MMP1 cross-reactivity is the liability.
A conventional Mmp13 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Mmp13 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Mmp13 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Mmp13 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mmp13 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mmp13 Knockout mouse models are available?
When Mmp13 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mmp13 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mmp13 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mmp13 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mmp13?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.