Mmp3 Knockout mouse models
Encoding stromelysin 1, Mmp3 is the mouse ortholog of human MMP3 and a secreted zinc endopeptidase with unusually broad substrate range, cleaving aggrecan core protein, laminin, fibronectin, nidogen, and type IV and IX collagen, and, importantly, acting as a physiologic activator of pro-MMP1, pro-MMP9, and pro-MMP13, which places it upstream in proteolytic cascades rather than only at the substrate face. Latency is maintained by the propeptide cysteine switch and released by plasmin or other proteases, with TIMP1 as dominant inhibitor, and expression is driven in synovial fibroblasts and chondrocytes by interleukin 1 beta and TNF, which is why Mmp3 nulls show altered severity in collagen induced arthritis and impaired contact hypersensitivity. Because deletion removes an activator as well as an enzyme, phenotypes are frequently indirect, so catalytically dead knockins and compound alleles with Mmp13 clarify causality, while fibroblast or chondrocyte restricted conditional deletion matches allele to endpoint.
Whole body loss of Mmp3 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Mmp3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Mmp3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Mmp3-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 200372 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Mmp3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mmp3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mmp3 Knockout mouse models are available?
When Mmp3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mmp3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mmp3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mmp3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mmp3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.