Mmp7 Knockout mouse models
Mmp7 is the mouse ortholog of human MMP7 and encodes matrilysin, the smallest matrix metalloproteinase, notable for lacking the hemopexin-like C-terminal domain entirely and for epithelial rather than stromal expression, most prominently in intestinal Paneth cells, airway epithelium, and glandular ducts. Beyond degrading fibronectin, laminin, and proteoglycans, its physiologically decisive activities are proteolytic maturation events: activation of pro-alpha defensins into bactericidal cryptdins in mouse small intestine, shedding of the E-cadherin ectodomain, release of soluble Fas ligand, and processing of syndecan bound chemokines. Mmp7 nulls consequently show impaired mucosal antimicrobial defense and altered wound repair rather than gross matrix failure, and the enzyme is an established progression biomarker in idiopathic pulmonary fibrosis. Match allele to question: conditional deletion with Vil1-Cre or Sftpc-CreER assigns the epithelial compartment, catalytically inactive knockins separate substrate processing from binding, and humanization is warranted where inhibitor selectivity or biomarker translation is the endpoint.
Global Mmp7 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Mmp7 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Mmp7 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Mmp7-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 190699 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Mmp7 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mmp7 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mmp7 Knockout mouse models are available?
When Mmp7 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mmp7 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mmp7 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mmp7 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mmp7?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.