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Msh2 Knockout mouse models
Msh2, the mouse ortholog of human MSH2, encodes the common subunit of both mismatch recognition complexes, pairing with Msh6 to form MutSalpha for base-base mispairs and small insertion or deletion loops and with Msh3 to form MutSbeta for larger loops; mismatch binding stimulates its Walker-motif ATPase, converting the heterodimer into a sliding clamp that recruits MutLalpha and licenses strand-specific excision, and the same machinery drives triplet repeat expansion and mediates the cytotoxicity of methylating agents. Germline biallelic loss causes constitutional mismatch repair deficiency and monoallelic loss causes Lynch syndrome, with microsatellite instability and hypermutation that confer immune checkpoint sensitivity. Nulls are viable, lymphoma-prone, and tolerant of temozolomide, so germline alleles are usable, while conditional deletion restricts hypermutation to a lineage of interest. ATPase separation-of-function knockins uncouple repair from damage signaling, and humanization supports variant classification. Msh6 and Msh3 are partners rather than substitutes, so no compensation rescues Msh2 loss.
Msh2 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Msh2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Msh2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Msh2-KO | KO | KO | DEVELOPING | KO 264098 | Inquire |
| The exon2-16 of Msh2 gene was deleted to generate Msh2 knockout mouse. | |||||
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Msh2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Msh2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Msh2 Knockout mouse models are available?
When Msh2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Msh2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Msh2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Msh2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Msh2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Msh2 Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.